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Part:BBa_K1122003:Design

Designed by: Lina Gasiūnaitė   Group: iGEM13_Edinburgh   (2013-08-13)


mTagBFP (BBa_K592100) under the control of lac promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The assembly of lac promoter, Linker1 with RBS and a start codon upstream the mTagBFP sequence and mTagBFP is contained in pSB1C3 vector. Since gene lacZ is known to be well expressed, Linker1 was designed to match lacZ upstream sequence containing an RBS and a start codon to enable protein expression.

Source

mTagBFP is based on the BioBrick BBa_K592100, lac promoter comes from genomic sequence of E. coli.

References

Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.